Anti-Nucleophosmin antibody, Rabbit monoclonal
SIGMA/SAB5500154 - recombinant, expressed in proprietary host, clone SP236, affinity isolated antibody
Product Type: Product-on-demand
Immunohistochemistry Anti-Nucleophosmin (NPM): Cat. No. SAB5500154: Immunohistochemistry of Nucleophosmin (NPM) in Human tonsil tissue with Nucleophosmin (NPM) antibody.
Immunoblotting Anti-Nucleophosmin (NPM): Cat. No. SAB5500154: Immunoblotting analysis of Nucleophosmin (NPM) in NIH/3T3 cell lysate with Nucleophosmin (NPM) antibody.
Flow Cytometry Anti-Nucleophosmin (NPM): Cat. No. SAB5500154: Flow cytometric analysis of Monoconal Rabbit Anti-Nucleophosmin (NPM) antibody in Jurkat (green) compare to negative control of Rabbit IgG (blue).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | SP236, monoclonal |
| conjugate | unconjugated |
| isotype | IgG |
| Quality Level | 100  |
| recombinant | expressed in proprietary host |
| shipped in | wet ice |
| species reactivity | human (tested) |
| species reactivity (predicted by homology) | bovine, rat, chicken, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 1:400 |
| immunoblotting: 1:400 | |
| immunohistochemistry: 1:100 | |
| UniProt accession no. | P06748  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Nucleophosmin (NPM) is a nucleolar protein for regulating ribosome biosynthesis and centrosome duplication. The NPM gene is frequently rearranged in various types of lymphomas such as anaplastic lymphoma kinase (ALK). A C-terminal mutation occurs in acute myelogenous leukemia (AML) and prevents localization to the nucleolus, resulting in cytoplasmic distribution. NPM is frequently overexpressed in diverse solid tumors such as carcinoma of the lung and breast, colon, prostate, stomach, lymphoid and myeloid tumors. |
| General description: | The NPM1 (nucleophosmin 1) gene is mapped to human chromosome 5q35.1. Nucleophosmin is a predominant phospho-nucleolar protein, that is mostly, localized to the nucleus or cytoplasm. |
| Immunogen: | Synthetic peptide derived from human nucleophosmin. |
| Physical form: | 0.1 ml rabbit monoclonal antibody purified by protein A/G in PBS/1% BSA buffer pH 7.6 with less than 0.1% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |