Anti-Vimentin antibody, Rabbit monoclonal
SIGMA/SAB5500191 - recombinant, expressed in proprietary host, clone SP20, tissue culture supernatant
Product Type: Product-on-demand
Immunohistochemistry Anti-Vimentin: Cat. No. SAB5500191: Immunohistochemistry of Vimentin in Human melanoma, sarcomas tissue with Vimentin antibody.
Immunoblotting Anti-Vimentin: Cat. No. SAB5500191: Immunoblotting analysis of Vimentin in HeLa cell lysate with Vimentin antibody.
Flow Cytometry Anti-Vimentin: Cat. No. SAB5500191: Flow cytometric analysis of Monoconal Rabbit Anti-Vimentin antibody in HeLa (green) compare to negative control of Rabbit IgG (blue).
| antibody form | tissue culture supernatant |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | SP20, monoclonal |
| conjugate | unconjugated |
| isotype | IgG |
| Quality Level | 100  |
| recombinant | expressed in proprietary host |
| shipped in | wet ice |
| species reactivity | human (tested) |
| species reactivity (predicted by homology) | bovine, rat, hamster, frog, chicken, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 1:100 |
| immunoblotting: 1:100 | |
| immunohistochemistry: 1:200 | |
| UniProt accession no. | P08670  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Vimentin is the main intermediate filament protein in mesenchymal cells. This protein is believed to be involved with the intracellular transport of proteins between the nucleus and plasma membrane. Vimentin stains sarcomas of neural, muscle and fibroblast origin, but carcinomas which are generally negative. Melanomas, lymphomas and vascular tumors may all stain for Vimentin. |
| Immunogen: | Recombinant human vimentin protein. |
| Physical form: | 0.1 ml rabbit monoclonal antibody supplied as tissue culture supernatant in TBS/1% BSA buffer pH 7.5 with less than 0.1% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |