Anti-Mouse IgG3 antibody, Rabbit monoclonal
SIGMA/SAB5600002 - recombinant, expressed in HEK 293 cells, clone RM218, purified immunoglobulin
Product Type: Chemical
ELISA ELISA of Mouse immunoglobulins shows RM218 reacts to both Mouse IgG3k and IgG3l. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM218 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.
ELISA A titer ELISA of Mouse IgG3. The plate was coated with different amounts of Mouse IgG3. A serial dilution of RM218 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM218, monoclonal | |
| concentration | ~1 mg/mL |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 380795   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 0.005-0.2 μg/mL |
| flow cytometry: suitable | |
| immunoblotting: 0.1-0.5 μg/mL | |
| immunocytochemistry: 0.5-2 μg/mL | |
| immunohistochemistry: 0.5-2 μg/mL | |
| immunoprecipitation (IP): suitable |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Purified mouse IgG3 whole molecule |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to the Fab region of mouse IgG3. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgM, IgA, IgE, human IgG, or rat IgG. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |