Anti-Mouse IgA antibody, Rabbit monoclonal
SIGMA/SAB5600003 - recombinant, expressed in HEK 293 cells, clone RM220, purified immunoglobulin
Product Type: Chemical
Immunoblotting Western blot of nonreduced(-) and reduced(+) Mouse IgA, using 0.5μg/mL of clone RM220. This antibody reacts to nonreduced IgA.
ELISA ELISA of Mouse immunoglobulins shows RM220 reacts to Mouse IgA. The plate was coated with 50 ng/well of different immunoglobulins. 200 ng/mL, 50 ng/mL, or 10 ng/mL of RM220 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.
ELISA A titer ELISA of Mouse IgA. The plate was coated with different amount of Mouse IgA. A serial dilution of RM220 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM220, monoclonal | |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 238447   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 0.005-0.2 μg/mL |
| flow cytometry: suitable | |
| immunoblotting: 0.5-2 μg/mL (non reduced only) | |
| immunocytochemistry: 0.5-2 μg/mL | |
| immunohistochemistry: 0.5-2 μg/mL | |
| immunoprecipitation (IP): suitable |
| Biochem/physiol Actions: | This antibody specifically reacts to mouse IgA. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG2c, IgG3, IgM, IgE, or human IgA. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Purified mouse IgA whole molecule |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |