Anti-Trimethyl-Histone H3 (Lys27) antibody, Rabbit monoclonal
SIGMA/SAB5600009 - recombinant, expressed in HEK 293 cells, clone RM175, purified immunoglobulin
Synonym: H3K27me3; Histone H3 (trimethyl K27)
Product Type: Chemical
Immunoblotting Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using RM175 at 1 mg/mL, showed a band of Histone H3 trimethylated at Lysine 27 (K27me3) in HeLa cells.
Chart RM175 specifically reacts to Histone H3 trimethylated at Lysine 27 (K27me3).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM175, monoclonal | |
| concentration | ~1 mg/mL |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 8350   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | trimethylation (Lys27) |
| technique(s) | ELISA: 0.5-1 μg/mL |
| immunohistochemistry: 0.5-2 μg/mL | |
| western blot: 1-2 μg/mL |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| Immunogen: | Synthetic peptide corresponding to Trimethyl-Histone H3 (Lys27) |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to Histone H3 trimethylated at Lysine 27 (K27me3). No cross reactivity with monomethylated Lysine 27 (K27me1) or dimethylated Lysine 27 (K27me2), or other methylations in histone H3. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |