Anti-Monomethyl-Histone H3 (Lys18) antibody, Rabbit monoclonal
SIGMA/SAB5600012 - recombinant, expressed in HEK 293 cells, clone RM167, purified immunoglobulin
Synonym: H3K18me1; Histone H3 (monomethyl K18)
Product Type: Chemical
Immunoblotting Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using RM167 at 1 mg/mL, showed a band of Histone H3 monomethylated at Lysine 18 (K18me1) in HeLa cells.
Chart RM167 specifically reacts to Histone H3 monomethylated at Lysine 18 (K18me1).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM167, monoclonal | |
| form | buffered aqueous glycerol solution |
| NCBI accession no. | 8350   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | monomethylation (Lys18) |
| technique(s) | ELISA: 0.2-1 μg/mL |
| western blot: 1-2 μg/mL |
| Biochem/physiol Actions: | Histones acts as structural scaffolds for DNA packaging. Histone H3 plays an important role in replication and DNA repair. Histone H3 is heavily post-translationally modified than the other members of histone family. Mutations in the gene leads to cerebellar high-grade gliomas. Phosphorylated histone H3 is implicated in the modulation of gene expression and in chromosome condensation/segregation. Monomethyl-histone H3 acts as a potent epigenetic mark for repressed euchromatin in Chlamydomonas. |
| Biochem/physiol Actions: | This antibody reacts to Histone H3 monomethylated at Lysine 18 (K18me1). No cross reactivity with dimethylated Lysine 18 (K18me2) or trimethylated Lysine 18(K18me3), or other methylations in histone H3. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Histone H3/ histone cluster 1 H3 family member a (HIST1H3A) is a core member of the histone family. HIST1H3A is encoded by the gene mapped to human chromosome 6p22.2. The encoded protein is composed of ~136 amino acids. |
| Immunogen: | Synthetic peptide corresponding to Monomethyl-Histone H3 (Lys18) |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |