Anti-Dimethyl Histone H3 (Lys79) antibody, Rabbit monoclonal
SIGMA/SAB5600014 - recombinant, expressed in HEK 293 cells, clone RM181, purified immunoglobulin
Synonym: H3K79me2; Histone H3 (dimethyl K79)
Product Type: Chemical
Immunohistochemistry Immunohistochemistry staining of HepG2 cells using Anti-Dimethyl-Histone H3 (Lys79) antibody, RM181.
Immunoblotting Western Blot of recombinant Histone H3.3 (1) and acid extracts of HeLa cells (2), using RM181 at 0.25 mg/mL, showed a band of Histone H3 dimethylated at Lysine 79 (K79me2) in HeLa cells.
Chromatin Immunoprecipitation ChIP performed on HeLa cells using H3K79me2 antibody (RM181, 5mg). Real-time PCR was performed using primers specific to the gene indicated.
Chart RM181 specifically reacts to Histone H3 dimethylated at Lysine 79 (K79me2).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM181, monoclonal | |
| concentration | ~1 mg/mL |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 8350   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | dimethylation (Lys79) |
| technique(s) | ChIP: 2-10 μg/mL |
| ELISA: 0.2-1 μg/mL | |
| immunohistochemistry: 0.1-1 μg/mL | |
| western blot: 0.25-1 μg/mL |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
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| Immunogen: | Synthetic peptide corresponding to Dimethyl-Histone H3 (Lys79) |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to Histone H3 dimethylated at Lysine 79 (K79me2). No cross reactivity with monomethylated Lysine 79(K79me1) or trimethylated Lysine 79 (K79me3), or other methylation in histone H3. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |