Anti-Monomethyl-Histone H4 (Arg3) antibody, Rabbit monoclonal
SIGMA/SAB5600017 - recombinant, expressed in HEK 293 cells, clone RM195, purified immunoglobulin
Synonym: H4R3me1; Histone H4 (monomethyl arg3)
Product Type: Chemical
Immunocytochemistry Immunocytochemical staining of HeLa cells using Anti-Monomethyl-Histone H4 (Arg3) Antibody (Clone RM195) (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Immunoblotting Western Blot of (1) acid extracts of HeLa cells; (2) recombinant Histone H4. Using Anti-Monomethyl-Histone H4 (Arg3) Antibody (Clone RM195) at 0.5 mg/mL, showed a band of Histone monomethylated at Arginine 3 in HeLa cells.
Chart RM195 specifically reacts to Histone H4 monomethylated at Arginine 3 (R3me1).
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM195, monoclonal | |
| concentration | ~1 mg/mL |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 121504   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | monomethylation (Arg3) |
| technique(s) | ELISA: 0.2-1 μg/mL |
| immunocytochemistry: 1-2 μg/mL | |
| western blot: 0.5-2 μg/mL |
| Biochem/physiol Actions: | This antibody reacts to Histone H4 monomethylated at Arginine 3 (R3me1). No cross reactivity with unmodified Arginine 3, asymmetric or symmetric dimethylated Arginine 3, and citrullinated Arginine 3 in Histone H4. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Synthetic peptide corresponding to the Monomethyl -Histone H4 (Arg3) |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |