Anti-Phospho-Histone H3 (pSer10) antibody, Rabbit monoclonal
SIGMA/SAB5600026 - recombinant, expressed in HEK 293 cells, clone RM163, purified immunoglobulin
Synonym: H3pSer10; Histone H3 (phospho ser10)
Product Type: Chemical
Immunocytochemistry Immunocytochemistry of HeLa cells using Phospho-Histone H3 (Ser10) Rabbit mAb RM163 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Immunoblotting Western Blot of acid extracts of HeLa cells treated or non-treated with Nocodazole. Using RM163 at 0.5 mg/mL, showed a band of Histone H3 phosphorylated at serine 10 in HeLa cells.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM163, monoclonal | |
| concentration | ~0.5 mg/mL |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 8350   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer10) |
| technique(s) | ELISA: 0.2- 1 μg/mL |
| immunocytochemistry: 0.5-2 μg/mL | |
| western blot: 0.5-2 μg/mL |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Synthetic peptide corresponding to Phospho-Histone H3 (Ser10) |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to Histone H3 phosphorylated at Serine 10. No cross reactivity with other phosphorylated histones. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |