Anti-Gamma H2AX (phospho-Ser139) antibody, Rabbit monoclonal
SIGMA/SAB5600038 - recombinant, expressed in HEK 293 cells, clone RM224, purified immunoglobulin
Synonym: H2AX Ser139 p; Histone H2AX (phospho ser139)
Product Type: Chemical
Immunocytochemistry Immunocytochemistry of HeLa cells using gamma H2AX Rabbit mAb RM224 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Immunoblotting Western Blot of acid extracts of HeLa cells treated or non-treated with UV. Using RM224 at 0.5 mg/mL, showed a band of Histone H2A.X phosphorylated at serine 139 in HeLa cells.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM224, monoclonal | |
| concentration | ~1 mg/mL |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 3014   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | ELISA: 0.2- 1 μg/mL |
| immunoblotting: 0.5-2 μg/mL | |
| immunocytochemistry: 0.5-2 μg/mL |
| Biochem/physiol Actions: | H2AX (H2A histone family member X) helps to maintain genome stability. It plays an important role in localisation and structuring of the repair focus. H2AX also plays an active role in supplying the active repair proteins. γH2AX participates in DSB repair. This protein serves as a signal and target of phosphorylation in the spreading phase. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | Histone H2AX (H2A histone family member X), a histone variant present in almost all eukaryotes, is coded by H2AFX gene. It is mapped to human chromosome 11q23.2–11q23.3. |
| Immunogen: | Synthetic peptide corresponding to Phospho-Histone H2AX (Ser139) |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to Histone H2A.X only when phosphorylated at serine 139. No cross reactivity with other phosphorylated histones. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |