Anti-Histone H2A antibody, Rabbit monoclonal
SIGMA/SAB5600042 - recombinant, expressed in HEK 293 cells, clone RM225, purified immunoglobulin
Product Type: Chemical
Immunocytochemistry Immunocytochemistry of HeLa cells, using Histone H2A Rabbit mAb RM225 (red). Actin filaments have been labeled with fluorescein phalloidin (green).
Immunoblotting Western Blot of recombinant Histone H2A, H2B, the whole cell lysates of HeLa, A375, SK-MEL-2, A431, and K562, using RM225 at 0.5 mg/mL.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | RM225, monoclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 3012   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | ELISA: 0.2- 1 μg/mL |
| immunoblotting: 0.5-2 μg/mL | |
| immunocytochemistry: 1-2 μg/mL |
| Biochem/physiol Actions: | This antibody reacts to the Histone H2A protein, independent of post-translational modifications. No cross reactivity with other histone proteins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Synthetic peptide corresponding to the C-terminus of human Histone H2A |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |