Anti-BRAF (V600E) antibody, Rabbit monoclonal
SIGMA/SAB5600047 - recombinant, expressed in HEK 293 cells, clone RM8, purified immunoglobulin
Synonym: Anti-B-RAF1; Anti-B-RAF1; Anti-B-raf; Anti-B-raf; Anti-BRAF-1; Anti-BRAF-1; Anti-BRAF1; Anti-BRAF1; Anti-NS7; Anti-NS7; Anti-RAFB1; Anti-RAFB1
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of formalin fixed and paraffin embedded melanoma tissue sections.
Immunoblotting Western Blot analysis of cell lysates prepared from cell lines expressing endogenous mutant (V600E) BRAF or wild type (WT) protein.
ELISA Sandwich ELISA performed on the lysates of 293T cells overexpressing BRAF V600E or BRAF wild type His-tag proteins, shows the specificity of RM8 to the V600E mutation. His-tag fusion proteins were captured by an Anti-His-Tag antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM8, monoclonal | |
| concentration | ~1 mg/mL |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 673   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | ELISA: 0.5-2 μg/mL |
| immunoblotting: 0.5-2 μg/mL | |
| immunocytochemistry: 0.5-5 μg/mL | |
| immunohistochemistry: 0.5-5 μg/mL | |
| UniProt accession no. | P15056 |
| Biochem/physiol Actions: | BRAF (B-Raf proto-oncogene) participates in signaling direct cell growth. It induces tumor growth by initiating the mitogen-activated protein kinase (MAPK) pathway in various tumors like cutaneous melanoma and carcinomas of the thyroid and colon. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | BRAF (B-Raf proto-oncogene) is a serine-threonine kinase, that codes for B-Raf protein. It is located on human chromosome 7q34. |
| Immunogen: | Peptide corresponding to BRAF V600E mutant |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |