Anti-Histone H3 K36M antibody, Rabbit monoclonal
SIGMA/SAB5600048 - recombinant, expressed in HEK 293 cells, clone RM193, purified immunoglobulin
Synonym: H3.3 K36M; H3K36M mutant
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3 K36M, stained with Anti-Histone H3 K36M clone RM193.
Immunohistochemistry Immunohistochemical staining of formalin fixed paraffin embedded (FFPE) Chondroblastoma tumor cell tissue sections. Image courtesy of Adrienne Flanagan, Department of Histopathology, RONH, Stanmore, Middlesex, UK
Immunoblotting Western Blot analysis of cell lysates prepared from 293T transfected with a DNA construct encoding wild type or K36M mutant proteins of Histone H3.1 and H3.3, using Anti-Histone H3 K36M clone RM193.
ELISA Sandwich ELISA performed on the lysates of 293T cells overexpressing Histone H3.3 K36M mutant or Histone H3.3 wild type His-tag proteins, shows the specificity of RM193 to the K36M mutant. The His-tag fusion proteins were captured by an Anti-His-Tag antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM193, monoclonal | |
| concentration | ~1 mg/mL |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 8350   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | ELISA: 0.5- 2 μg/mL |
| immunoblotting: 0.5-2 μg/mL | |
| immunocytochemistry: 0.5-5 μg/mL | |
| immunohistochemistry: 0.5-5 μg/mL |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | Peptide corresponding to Histone H3 K36M mutant |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to the Histone H3 K36M mutant. No cross reactivity with wild type Histone H3. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352200 |