Anti-p38 MAPK Phospho (pT180/pY182) antibody, Rabbit monoclonal
SIGMA/SAB5600057 - recombinant, expressed in HEK 293 cells, clone RM243, purified immunoglobulin
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using Anti-Phospho-p38 MAPK (Thr180/Tyr182) RM243 at a 1:1000 dilution.
Immunoblotting Western Blot of HeLa cell lysates, treated or nontreated with anisomycin, using Anti-Phospho-p38 MAPK (Thr180/Tyr182) RM243 at a 1:2000 dilution, showed a band of Phospho-p38 MAPK (~38 kDa) in anisomycin-treated HeLa cells.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM243, monoclonal | |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 1432   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pThr180/pTyr182) |
| technique(s) | immunoblotting: 1:1000-1:2000 |
| immunohistochemistry: 1:500-1:1000 |
| Application: | Anti-p38 MAPK Phospho (pT180/pY182) antibody, Rabbit monoclonal has been used for immunodetection. |
| Biochem/physiol Actions: | Mitogen activated protein kinase (MAPK) participates in transdifferentiation of adipocytes in myoblast by regulating adipogenic and adipolysis metabolism. p38 MAPK is associated with various biological processes as such as cell proliferation and differentiation, cell adhesion, migration. It mediates pro-apoptotic signal during apoptosis, contributing either to cell death or survival.. p38 MAPK is known to mediate a number of metabolic pathways by controlling transcription, mRNA stability, chromatin remodelling and protein synthesis. During initial process of tumorigenesis, It acts as a tumor suppressor, while it might promote metastasis in the later stages of the same. p38 MAPK gets activated in response to growth factors, oxidative stress, proinflammatory cytokines and DNA damage. |
| Biochem/physiol Actions: | This antibody reacts to p38 MAPK only when dual phosphorylation at Thr180 and Tyr182 is observed. There is no cross-reactivity with p38 MAPK without dual phosphorylation at Thr180 and Tyr182. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The mitogen activated protein kinase (MAPK) gene codes for a serine/threonine protein kinase which is found to be expressed in majority of cells. The MAPK gene is mapped to human chromosome 6p21.31 and the encoded protein consists of 90 to 360 amino acids residues. MAPKs are divided into three subtypes : extracellular signal-regulated kinases (ERK), c-Jun amino-terminal kinases (JNK) and p38 MAPK (p38). |
| Immunogen: | Synthetic phospho-peptide corresponding to human Phospho-p38 MAPK (Thr180/Tyr182) |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |