Anti-Phospho-akt (ser473) antibody, Rabbit monoclonal
SIGMA/SAB5600064 - recombinant, expressed in HEK 293 cells, clone RM251, purified immunoglobulin
Synonym: phosphorylation at Ser473
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of formalin fixed and paraffin embedded Human breast cancer tissue sections using Anti-Phospho-Akt (Ser473) RM251 at a 1:400 dilution.
Immunoblotting Western Blot of lysates from 293 cells either, grown in medium with serum, serum starved, or insulin treated. Using Anti-Phospho-Akt (Ser473) RM251 at a 1:1000 dilution or Anti-Akt1 RM252 at a 1:1000 dilution.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM251, monoclonal | |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 207   |
| Quality Level | 100 |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | phosphorylation (pSer473) |
| technique(s) | immunoblotting: 1:1000-1:2000 |
| immunohistochemistry: 1:200-1:500 |
| Biochem/physiol Actions: | AKT1 (AKT serine/threonine kinase 1) controls the survival of cells and anti-apoptotic actions that attack the pathogenesis of several cancers, hence it plays a crucial role in tumorigenesis. In mice, AKT1 is essential for normal development. |
| Biochem/physiol Actions: | This antibody reacts to Akt only when phosphorylated at Ser473. There is no cross-reactivity with Akt without phosphorylation at Ser473. This antibody may also react to bovine, mouse or rat Phospho-Akt (Ser473), as predicted by immunogen homology |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | AKT1 (AKT serine/threonine kinase 1) is also called as v-akt murine thymoma viral oncogene homolog 1 and PKB α (protein kinase B). It is expressed in liver,muscle and adipocytes. AKT1 gene is mapped to human chromosome 14q32. It is a member of AKT family that has an amino-terminal pleckstrin homology (PH) domain, a short α helical linker and a carboxyl-terminal kinase domain. |
| Immunogen: | Synthetic peptide corresponding to human Phospho-Akt (Ser473) |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |