Anti-Histone H3 K27M antibody, Rabbit Monoclonal
SIGMA/SAB5600095 - recombinant, expressed in HEK 293 cells, clone RM192, purified immunoglobulin
Synonym: Anti-Histone H3 K27M; Histone H3 K27M Detection; Rabbit Anti-Histone H3 K27M
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of formalin fixed and paraffin embedded 293T cells transfected with a DNA construct encoding Histone H3 K27M mutant, stained with 0.01 μg/mL of Anti-Histone H3K27M Rabbit monoclonal antibody clone RM192.
Immunohistochemistry Immunostaining of brain tumor tissue sections using both EDTA and Citrate pretreatment methods. Clinically validated to be Positive for the H3.3 K27M Mutation by Sanger sequencing. Image courtesy of Sebastian Brandner MD, Division of Neuropathology and Dept. of Neurodegenerative Disease, UCL Institute of Neurology, London, UK.
Immunohistochemistry Immunostaining of brain tumor tissue sections using both EDTA and Citrate pretreatment methods. Clinically validated to be Negative for the H3.3 K27M Mutation by Sanger sequencing. Image courtesy of Sebastian Brandner MD, Division of Neuropathology and Dept. of Neurodegenerative Disease, UCL Institute of Neurology, London, UK.
Western Blotting Western Blot analysis of cell lysates prepared from 293T transfected with a DNA construct encoding wild type (WT) or K27M mutant proteins of Histone H3.3, using 0.01 μg/mL of Anti-Histone H3 K27M Rabbit monoclonal antibody clone RM192.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM192, monoclonal | |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 8350   |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | mutation (Lys27Met) |
| technique(s) | ELISA: 0.5-2 μg/mL |
| immunocytochemistry: 0.005-0.1 μg/mL | |
| immunohistochemistry: 0.005-0.1 μg/mL | |
| western blot: 0.01-1 μg/mL |
| Biochem/physiol Actions: | This antibody reacts to the Histone H3 K27M mutant. No cross reactivity with wild type Histone H3. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| Immunogen: | A peptide corresponding to Histone H3 K27M mutant. |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |