Anti-HA-Tag antibody, Rabbit Monoclonal
SIGMA/SAB5600116 - recombinant, expressed in HEK 293 cells, clone RM305, purified immunoglobulin
Synonym: Human influenza hemagglutinin amino acids 98-106
Product Type: Chemical
Immunohistochemistry Immunohistochemical staining of formalin fixed and paraffin embedded LNCaP cells overexpressing HA-tag Bag1 protein, using Anti-HA-tag Rabbit monoclonal antibody (clone RM305) at 0.01 mg/mL.
Western Blotting Western blot of 293T cells transfected (+) or non-transfected (-) with a DNA construct encoding HA-Tag KRas fusion protein, using Anti-HA-tag Rabbit monoclonal antibody (clone RM305) at 0.1 mg/mL.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM305, monoclonal | |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| Quality Level | 100  |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | flow cytometry: 0.1-1 μg/mL |
| immunocytochemistry: 0.1-1 μg/mL | |
| immunohistochemistry: 0.01-0.5 μg/mL | |
| immunoprecipitation (IP): 0.5-2 μg/mL | |
| western blot: 0.1-1 μg/mL |
| Application: | Anti-HA-Tag antibody, Rabbit Monoclonal has been used in western blotting. |
| Biochem/physiol Actions: | Hemagglutinin (HA) regulates membrane fusion and receptor-binding functions during viral entry and infection. The virus gains entry into the host cell via endocytosis and successive membrane fusion mediated by the HA antigen. HA plays a crucial role in viral pathogenesis and host response to viral infection. |
| Biochem/physiol Actions: | This antibody reacts to recombinant proteins containing a HA-Tag fused to either the amino or carboxy terminus. No cross reactivity with other endogenous protein. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Features and Benefits: | Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more. |
| General description: | The hemagglutinin (HA) gene is mapped to segment 4 of Influenza B viral genome. HA is a dominant antigen present on the influenza viral surface. It is a homotrimer and each monomer is produced as a single polypeptide. |
| Immunogen: | A peptide corresponding to HA-tag (Human influenza hemagglutinin amino acids 98-106). |
| Physical form: | Solution in phosphate buffered saline containing 50% glycerol, 1% BSA and 0.09% sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |