Anti-Phosphotyrosine antibody, Rabbit monoclonal
SIGMA/SAB5600274 - recombinant, expressed in HEK 293 cells, clone RM111, purified immunoglobulin
Synonym: p-Tyr; tyrosine-
Product Type: Chemical
Immunocytochemistry Immunocytochemistry of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/500 dilution (followed by a PE conjugated secondary antibody, red) and DAPI (blue).
Western Blotting Western blot of serum-starved A431 cells nontreated or treated with EGF, using Clone RM111 at 1/5000 dilution. The blot was exposed to the film at different time points.
Immunoprecipitation Immunoprecipitation of EGF-treated A431 cells by Clone RM111 at 1/1000 dilution, was blotted with an Anti-EGFR Rabbit monoclonal antibody. (1) Whole lysate control; (2) IP by Rabbit IgG control; (3) IP by Clone RM111.
Multiplex Luminex analysis of Clone RM111’s reaction against phosphorylated or nonphosphorylated Serine, Threonine, and Tyrosine. Clone RM111 reacts only to phosphorylated Tyrosine.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM111, monoclonal | |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | all |
| storage temp. | −20°C |
| technique(s) | ChIP: 1:200-1:1000 |
| ELISA: suitable (assay dependent concentration) | |
| flow cytometry: suitable (assay dependent concentration) | |
| immunocytochemistry: 1:200-1:500 | |
| immunohistochemistry: 1:200—1:500 | |
| immunoprecipitation (IP): 1:200-1:1000 | |
| western blot: 1:1000-1:5000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Phosphotyrosine-BSA conjugate |
| Physical form: | Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |