Anti-Acetylated-Lysine antibody, Rabbit monoclonal
SIGMA/SAB5600275 - recombinant, expressed in HEK 293 cells, clone RM101, purified immunoglobulin
Synonym: pan acetyl Lysine
Product Type: Chemical
Immunocytochemistry Immunocytochemical staining of HeLa cells using Anti-Acetylated Lysine antibody (RM101) (red). Actin filaments was labeled with fluorescein phalloidin (green), and nucleus stained with DAPI (blue).
Western Blotting Western blot of HeLa cells nontreated or treated with Trichostatin A (TSA), using Clone RM101 at 1/2000 dilution.
Immunoprecipitation Immunoprecipitation of A431 cell lysates, using clone RM101 at 1/500 dilution, was blotted with Anti-PTEN Mouse monoclonal antibody.
Multiplex Anti-Acetylated Lysine Rabbit Monoclonal Antibody clone RM101 recognizes acetylated lysine in peptides with different sequences. It does not react with unmodified lysine.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RM101, monoclonal | |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | all |
| storage temp. | −20°C |
| technique(s) | ChIP: 1:100-1:500 |
| ELISA: suitable (assay dependent concentration) | |
| flow cytometry: suitable (assay dependent concentration) | |
| immunocytochemistry: 1:100-1:500 | |
| immunohistochemistry: 1:100—1:500 | |
| immunoprecipitation (IP): 1:100-1:500 | |
| western blot: 1:500 —1:2000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Acetyllysine -BSA |
| Physical form: | Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to lysine-acetylated proteins. No cross reactivity with nonacetylated lysine, and lysine with other modification. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |