Anti-Human Ig Light Chains-Biotin antibody, Rabbit monoclonal
SIGMA/SAB5600276 - recombinant, expressed in HEK 293 cells, clone RM129, purified immunoglobulin
Synonym: immunoglobutin light chain
Product Type: Chemical
ELISA ELISA of Human immunoglobulins shows RM129 reacts to both kappa and lambda light chains of Human immunoglobulins. No cross reactivity with Mouse, Rat, or Goat immunoglobulin light chain. The plate was coated with 50 ng/well of different immunoglobulins. 100 ng/mL or 20 ng/mL of RM129 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Rabbit IgG as the secondary antibody.
ELISA A titer Sandwich ELISA using Biotinylated RM129 as the detection antibody. The plate, coated with the capture antibody Anti-Human IgG RM116, was loaded with different amounts of Human IgG. A serial dilution of Biotin RM129 was used as the detection antibody, followed with an alkaline phosphatase conjugated streptavidin.
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| clone | recombinant monoclonal |
| RM129, monoclonal | |
| conjugate | biotin conjugate |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| NCBI accession no. | 3514   |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | ELISA: 0.05-0.5 μg/mL |
| flow cytometry: suitable (assay dependent concentration) | |
| immunocytochemistry: 0.5-2 μg/mL | |
| immunohistochemistry: 0.5—2 μg/mL | |
| UniProt accession no. | P01834 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Human IgG |
| Physical form: | Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to both kappa and lambda light chains of human immunoglobulins. The antibody does not react to monkey (Cyno or Rhesus) IgG, mouse IgG, rat IgG, or goat IgG. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |