Anti-Mouse IgG Fc antibody, Goat monoclonal
SIGMA/SAB5600283 - recombinant, expressed in HEK 293 cells, clone RMG06, purified immunoglobulin
Synonym: Fc region of Mouse IgG
Product Type: Chemical
ELISA ELISA of Mouse immunoglobulins shows RMG06 reactsto the Fc region of Mouse IgG1, IgG2a, and IgG2b, and very slightly to IgG3; no cross reactivity with IgM, IgA, IgE, Human IgG, Rat IgG, and Rabbit IgG. The plate was coated with 50 ng/well of different immunoglobulins. 500 ng/mL, 200 ng/mL, or 50 ng/mL of RMG06 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Goat IgG as the secondary antibody.
ELISA A titer ELISA of Mouse IgG. The plate was coated with different amounts of Mouse IgG. A serial dilution of RMG06 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Goat IgG as the secondary antibody.
| antibody form | purified immunoglobulin |
| antibody product type | primary antibodies |
| clone | recombinant monoclonal |
| RMG06, monoclonal | |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | mouse |
| storage temp. | −20°C |
| technique(s) | ELISA: 0.05-1 μg/mL |
| flow cytometry: suitable (assay dependent concentration) | |
| immunocytochemistry: 0.5-2 μg/mL | |
| immunohistochemistry: 0.5—2 μg/mL | |
| immunoprecipitation (IP): suitable (assay dependent concentration) | |
| western blot: 0.1— 0.5 μg/mL |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Mouse IgG |
| Physical form: | Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to the Fc region of Mouse IgG, including IgG1, IgG2a, and IgG2b. It shows very slight reaction with mouse IgG3. No cross reactivity with IgM, IgA, IgE, human IgG, rat IgG, or rabbit IgG. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |