Anti-Rabbit IgG-Biotin antibody, Goat monoclonal
SIGMA/SAB5600287 - recombinant, expressed in HEK 293 cells, clone RMG03, purified immunoglobulin
Product Type: Chemical
ELISA ELISA of IgGs from different species shows RMG03 reacts to Rabbit IgG; no cross reactivity with Human IgG, Rat IgG, or Mouse IgG. The plate was coated with 50 ng/well of different IgG. 500 ng/mL, 200 ng/mL, or 50 ng/mL of RMG03 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Goat IgG as the secondary antibody.
ELISA A titer ELISA of Rabbit IgG. The plate was coated with different amounts of Rabbit IgG. A serial dilution of RMG03 was used as the primary antibody. An alkaline phosphatase conjugated Anti-Goat IgG as the secondary antibody.
| antibody form | purified immunoglobulin |
| antibody product type | secondary antibodies |
| clone | recombinant monoclonal |
| RMG03, monoclonal | |
| conjugate | biotin conjugate |
| form | buffered aqueous glycerol solution |
| isotype | IgG |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| species reactivity | rabbit |
| storage temp. | −20°C |
| technique(s) | ELISA: 0.01-0.5 μg/mL |
| immunocytochemistry: 0.5-2 μg/mL | |
| immunohistochemistry: 0.5—2 μg/mL | |
| western blot: 0.1— 0.5 μg/mL |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | Rabbit IgG |
| Physical form: | Solution in phosphate buffered saline containing 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide. |
| Specificity: | This antibody reacts to Rabbit IgG. No cross reactivity with human IgG, rat IgG, or mouse IgG. |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |