Anti-KDM1 antibody produced in rabbit
SIGMA/SAB5700057
Synonym: AOF2; BHC110; CPRF; KDM1; KDM1; KDM1A; LSD1
Product Type: Chemical
Enhanced Validation-By Genetic Strategies Western blot analysis of extracts from normal (control) and KDM1 knockout (KO) HeLa cells, using KDM1 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 90 s.
Immunofluorescence Immunofluorescence analysis of A549 cells using KDM1 antibody.
Western Blotting Western blot analysis of extracts of various cell lines, using KDM1 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004).
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg KDM1 antibody. Western blot was performed from the immunoprecipitate using KDM1 antibody at a dilution of 1:500.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 3.01 mg/ml |
| form | buffered aqueous solution |
| mol wt | 92 |
| 95 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:100 |
| immunoprecipitation (IP): 1:50-1:100 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | O60341  |
| General description: | This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |