Anti-NUDT1 antibody produced in rabbit
SIGMA/SAB5700089
Synonym: MTH1; NUDT1
Product Type: Chemical
Enhanced Validation-By Genetic Strategies Western blot analysis of extracts from normal (control) and NUDT1 knockout (KO) 293T cells, using NUDT1 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Immunohistochemistry Immunohistochemistry of paraffin-embedded human gastric cancer using NUDT1 antibody at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using NUDT1 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of 293T cells using 1 μg NUDT1 antibody. Western blot was performed from the immunoprecipitate using NUDT1 antibody at a dilution of 1:1,000.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.91 mg/ml |
| form | buffered aqueous solution |
| mol wt | 17 |
| 19 | |
| 20 | |
| 22 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| immunoprecipitation (IP): 1:20-1:50 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P36639  |
| General description: | Misincorporation of oxidized nucleoside triphosphates into DNA/RNA during replication and transcription can cause mutations that may result in carcinogenesis or neurodegeneration. The protein encoded by this gene is an enzyme that hydrolyzes oxidized purine nucleoside triphosphates, such as 8-oxo-dGTP, 8-oxo-dATP, 2-hydroxy-dATP, and 2-hydroxy rATP, to monophosphates, thereby preventing misincorporation. The encoded protein is localized mainly in the cytoplasm, with some in the mitochondria, suggesting that it is involved in the sanitization of nucleotide pools both for nuclear and mitochondrial genomes. Several alternatively spliced transcript variants, some of which encode distinct isoforms, have been identified. Additional variants have been observed, but their full-length natures have not been determined. A single-nucleotide polymorphism that results in the production of an additional, longer isoform (p26) has been described. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-179 of human NUDT1 (NP_945192.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |