Anti-Acetyl-Histone H4-K5 antibody produced in rabbit
SIGMA/SAB5700108
Synonym: FO108; H4; H4/n; H4F2; H4FN; HIST1H4A; HIST2H4; HIST2H4A; Histone H4
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using H4K5AC antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human tonsil using H4K5AC antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human stomach using H4K5AC antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using H4K5AC antibody at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of various cell lines, using Acetyl-Histone H4-K5 antibody at 1:1,000 dilution. HeLa cells were treated by TSA (1uM) for 18 hours. C6 cells were treated by TSA (1uM) for 18 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 30 s.
Chromatin Immunoprecipitation Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Acetyl-Histone H4-K5 antibody and Rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.28 mg/ml |
| form | buffered aqueous solution |
| mol wt | 11 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | ChIP: 1:20-1:100 |
| immunofluorescence: 1:50-1:200 | |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:200-1:2000 | |
| UniProt accession no. | P62805  |
| General description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H4 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is found in a histone cluster on chromosome 1. This gene is one of four histone genes in the cluster that are duplicated; this record represents the centromeric copy. |
| Immunogen: | A synthetic acetylated peptide around K5 of human Histone H4 (NP_001029249.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |