Anti-XPA antibody produced in rabbit
SIGMA/SAB5700125
Synonym: XP1; XPA; XPAC
Product Type: Chemical
Enhanced Validation-By Genetic Strategies Western blot analysis of extracts from normal (control) and XPA knockout (KO) HeLa cells, using XPA antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 15 s.
Immunofluorescence Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using XPA antibody. Green:GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence Immunofluorescence analysis of A549 cells using XPA antibody.
Western Blotting Western blot analysis of extracts of various cell lines, using XPA antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.02 mg/ml |
| form | buffered aqueous solution |
| mol wt | 31 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:100 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P23025  |
| General description: | This gene encodes a zinc finger protein involved in DNA excision repair. The encoded protein is part of the NER (nucleotide excision repair) complext which is responsible for repair of UV radiation-induced photoproducts and DNA adducts induced by chemical carcinogens. Mutations in this gene are associated with xeroderma pigmentosum complementation group A. Alternatively spliced transcript variants have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-273 of human XPA (NP_000371.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |