Anti-MonoMethyl-Histone H2B-K5 antibody produced in rabbit
SIGMA/SAB5700139
Synonym: GL105; H2B; H2B.1; H2BFQ; H2BGL105; H2BQ; HIST2H2BE; Histone H2B
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of C6 cells using MonoMethyl-Histone H2B-K5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H2B-K5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U-2 OS cells using MonoMethyl-Histone H2B-K5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH-3T3 cells using MonoMethyl-Histone H2B-K5 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of C6 cells, using MonoMethyl-Histone H2B-K5 antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 180 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.84 mg/ml |
| form | buffered aqueous solution |
| mol wt | 13 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q16778  |
| General description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif. The protein has antibacterial and antifungal antimicrobial activity. [provided by RefSeq, Aug 2015] |
| Immunogen: | A synthetic peptide of Human H2BK5me1. |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |