Anti-CSNK1E antibody produced in rabbit
SIGMA/SAB5700142
Synonym: CKIepsilon; CSNK1E; HCKIE
Product Type: Chemical
Enhanced Validation-By Genetic Strategies Western blot analysis of extracts from normal (control) and CSNK1E knockout (KO) HeLa cells, using CSNK1E antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 5 s.
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat pancreas using CSNK1E antibody at dilution of 1:200 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using CSNK1E antibody at dilution of 1:200 (40x lens).
Immunofluorescence Immunofluorescence analysis of HeLa cells using CSNK1E antibody. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using CSNK1E antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 90 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.21 mg/ml |
| form | buffered aqueous solution |
| mol wt | 47 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| immunoprecipitation (IP): 1:50-1:100 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P49674  |
| General description: | The protein encoded by this gene is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 247-416 of human CSNK1E (NP_689407.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |