Anti-SMYD3 antibody produced in rabbit
SIGMA/SAB5700157
Synonym: KMT3E; SMYD3; ZMYND1; ZNFN3A1; bA74P14.1
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of C6 cells using SMYD3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of HeLa cells using SMYD3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of L929 cells using SMYD3 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using SMYD3 antibody at 1:3,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 90 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.82 mg/ml |
| form | buffered aqueous solution |
| mol wt | 29 |
| 42 | |
| 49 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q9H7B4  |
| General description: | This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 50-150 of human SMYD3 (NP_001161212.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |