Anti-Acetyl-Histone H3-K18 antibody produced in rabbit
SIGMA/SAB5700245
Synonym: H3/j; H3FJ; HIST1H3J
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat ovary using H3K18ac antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using H3K18ac antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human stomach using H3K18ac antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human gastric cancer using H3K18ac antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse liver using H3K18ac antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using H3K18ac antibody at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of C6 cells using Acetyl-Histone H3-K18 antibody at dilution of 1:100. C6 cells were treated by TSA (1 μM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K18 antibody at dilution of 1:100. HeLa cells were treated by TSA (1 μM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K18 antibody at dilution of 1:100. NIH/3T3 cells were treated by TSA (1 μM) at 37 °C for 18 hours. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K18 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 90 s.
Dot Blot Dot-blot analysis of all sorts of methylation peptides using Acetyl-Histone H3-K18 antibody at 1:1,000 dilution.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.57 mg/ml |
| form | buffered aqueous solution |
| mol wt | 15 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | ChIP: 1:50-1:100 |
| immunofluorescence: 1:500-1:1000 | |
| immunohistochemistry: 1:200-1:500 | |
| immunoprecipitation (IP): 1:200-1:500 | |
| western blot: 1:1000-1:3000 | |
| UniProt accession no. | P68431  |
| General description: | Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. |
| Immunogen: | A synthetic acetylated peptide corresponding to residues surrounding K18 of human H3 |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |