Anti-YBX1 antibody produced in rabbit
SIGMA/SAB5700257
Synonym: BP-8; CBF-A; CSDA2; CSDB; DBPB; EFI-A; MDR-NF1; NSEP-1; NSEP1; YB-1; YB1; YBX1
Product Type: Chemical
Enhanced Validation-By Genetic Strategies Western blot analysis of extracts from normal (control) and YBX1 knockout (KO) HeLa cells, using YBX1 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 5 s.
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat testis using YBX1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse testis using YBX1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon carcinoma using YBX1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of mouse colon using YBX1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of mouse colone artery using YBX1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of mouse brain using YBX1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of HepG2 cells, using YBX1 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.29 mg/ml |
| form | buffered aqueous solution |
| mol wt | 35 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:100-1:200 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P67809  |
| General description: | This gene encodes a highly conserved cold shock domain protein that has broad nucleic acid binding properties. The encoded protein functions as both a DNA and RNA binding protein and has been implicated in numerous cellular processes including regulation of transcription and translation, pre-mRNA splicing, DNA reparation and mRNA packaging. This protein is also a component of messenger ribonucleoprotein (mRNP) complexes and may have a role in microRNA processing. This protein can be secreted through non-classical pathways and functions as an extracellular mitogen. Aberrant expression of the gene is associated with cancer proliferation in numerous tissues. This gene may be a prognostic marker for poor outcome and drug resistance in certain cancers. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on multiple chromosomes. |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human YBX1 (NP_004550.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |