Anti-Phospho-ABL1-Y245 antibody produced in rabbit
SIGMA/SAB5700276
Synonym: ABL; ABL1; CHDSKM; JTK7; bcr/abl; c-ABL; c-ABL1; c-Abl; p150; v-abl
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat kidney using Phospho-ABL1-Y245 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human liver cancer using Phospho-ABL1-Y245 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse liver using Phospho-ABL1-Y245 antibody at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of C6 cells, using Phospho-ABL1-Y245 pAb at 1:1,000 dilution or ABL1 antibody. C6 cells were treated by CIP (20 μL/400 μL) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 3S.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.09mg/ml |
| form | buffered aqueous solution |
| mol wt | 122 |
| 124 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P00519  |
| General description: | This gene is a protooncogene that encodes a protein tyrosine kinase involved in a variety of cellular processes, including cell division, adhesion, differentiation, and response to stress. The activity of the protein is negatively regulated by its SH3 domain, whereby deletion of the region encoding this domain results in an oncogene. The ubiquitously expressed protein has DNA-binding activity that is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function. This gene has been found fused to a variety of translocation partner genes in various leukemias, most notably the t(9;22) translocation that results in a fusion with the 5′ end of the breakpoint cluster region gene (BCR; MIM:151410). Alternative splicing of this gene results in two transcript variants, which contain alternative first exons that are spliced to the remaining common exons. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding Y245 of human ABL1 |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |