Anti-Phospho-APP-T668 antibody produced in rabbit
SIGMA/SAB5700277
Synonym: Aβ42; AAA; ABETA; ABPP; AD1; APP; APPI; Amyloid beta A4; CTFgamma; CVAP; PN-II; PN2; preA4
Product Type: Chemical
Western Blotting Western blot analysis of extracts of various cell lines, using Phospho-APP-T668 antibody at 1:1,000 dilution. Mouse brain lysate were treated by CIP (20 μL CIP for each 400 μL cell lysate) at 37 °C for 1 hour. Rat brain lysate were treated by CIP (20 μL CIP for each 400 μL cell lysate) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.81 mg/ml |
| form | buffered aqueous solution |
| mol wt | 34 |
| 72-86 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P05067  |
| General description: | This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. In addition, two of the peptides are antimicrobial peptides, having been shown to have bacteriocidal and antifungal activities. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T668 of human APP |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |