Anti-Phospho-ATM-S1981 antibody produced in rabbit
SIGMA/SAB5700279
Synonym: AT1; ATA; ATC; ATD; ATDC; ATE; ATM; TEL1; TELO1
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of MCF-7 cells using Phospho-ATM-S1981 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U2OS cells using Phospho-ATM-S1981 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of U2OS cells using Phospho-ATM-S1981 antibody. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of 293T cells, using Phospho-ATM-S1981 antibody at 1:2,000 dilution. 293T cells were treated by UV for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.02 mg/ml |
| form | buffered aqueous solution |
| mol wt | 350 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | Q13315  |
| General description: | The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. |
| Immunogen: | A synthetic phosphorylated peptide around S1981 of human ATM (NP_000042.3). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |