Anti-Phospho-CDK1-T14 antibody produced in rabbit
SIGMA/SAB5700285
Synonym: CDC2; CDC28A; CDK1; P34CDC2
Product Type: Chemical
Immunofluorescence Immunofluorescence analysis of MCF-7 cells using Phospho-CDK1-T14 antibody. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of HT-29 and HeLa cells, using Phospho-CDK1-T14 antibody at 1:1,000 dilution. HT-29 cells were treated by serum-starvation overnight. HeLa cells were treated by UV for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10min.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.56 mg/ml |
| form | buffered aqueous solution |
| mol wt | 27 |
| 34 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:100 |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P06493  |
| General description: | The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around T14 of human CDK1 (NP_001777.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |