Anti-Phospho-CHEK1-S280 antibody produced in rabbit
SIGMA/SAB5700286
Synonym: CHEK1; CHK1
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat heart using Phospho-CHEK1-S280 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human placenta using Phospho-CHEK1-S280 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse brain using Phospho-CHEK1-S280 antibody at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of MCF-7 cells using Phospho-CHEK1-S280 antibody. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of HT-29 cells, using Phospho-CHEK1-Ser280 antibody at 1:1,000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.66 mg/ml |
| form | buffered aqueous solution |
| mol wt | 43 |
| 50 | |
| 54 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:200-1:2000 | |
| UniProt accession no. | O14757  |
| General description: | The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene. |
| Immunogen: | A synthetic phosphorylated peptide around S280 of human CHEK1 (NP_001265.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |