Anti-Phospho-ATF2-T71 antibody produced in rabbit
SIGMA/SAB5700288
Synonym: ATF2; CRE-BP1; CREB-2; CREB2; HB16; TREB7
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat pancreas using Phospho-ATF2-T71 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-ATF2-T71 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-ATF2-T71 antibody at dilution of 1:100 (40x lens).
Immunofluorescence Immunofluorescence analysis of MCF-7 cells using Phospho-ATF2-T71 antibody. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Phospho-ATF2-T71 antibody at 1:1,000 dilution. HeLa cells were treated by Anisomycin (25 μg/mL) for 30 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25 μg/mL) for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 3.24 mg/ml |
| form | buffered aqueous solution |
| mol wt | 13- 15 |
| 23-24 | |
| 35-54 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:100 |
| immunohistochemistry: 1:50-1:100 | |
| western blot: 1:500-1:1000 | |
| UniProt accession no. | P15336  |
| General description: | This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell′s DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene |
| Immunogen: | A synthetic phosphorylated peptide around T71 of human ATF2 (NP_001871.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |