Anti-Phospho-VASP-S157 antibody produced in rabbit
SIGMA/SAB5700441
Synonym: VASP
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using Phospho-VASP-S157 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded human colon using Phospho-VASP-S157 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse lung using Phospho-VASP-S157 antibody at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of A431 cells, using Phospho-VASP-S157 antibody at 1:2,000 dilution. A431 cells were treated by Forskolin (10 μM) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Immunoprecipitation Immunoprecipitation analysis of 200 μg extracts of A-431 cells, using 3 μg Phospho-VASP-S157 pAb at a dilution of 1:1,000. A-431 cells were treated by Forskolin (10 μM) at 37 °C for 30 minutes after serum-starvation overnight.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.35 mg/ml |
| form | buffered aqueous solution |
| mol wt | 39 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| immunoprecipitation (IP): 1:50-1:100 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P50552  |
| General description: | Vasodilator-stimulated phosphoprotein (VASP) is a member of the Ena-VASP protein family. Ena-VASP family members contain an EHV1 N-terminal domain that binds proteins containing E/DFPPPPXD/E motifs and targets Ena-VASP proteins to focal adhesions. In the mid-region of the protein, family members have a proline-rich domain that binds SH3 and WW domain-containing proteins. Their C-terminal EVH2 domain mediates tetramerization and binds both G and F actin. VASP is associated with filamentous actin formation and likely plays a widespread role in cell adhesion and motility. VASP may also be involved in the intracellular signaling pathways that regulate integrin-extracellular matrix interactions. VASP is regulated by the cyclic nucleotide-dependent kinases PKA and PKG. |
| Immunogen: | A synthetic phosphorylated peptide around S157 of human VASP (NP_003361.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |