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Anti-Phospho-PPP1R12A-S507 antibody produced in rabbit

SIGMA/SAB5700445

Synonym: M130; MBS; MYPT1; MYPT1; PPP1R12A

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-SAB5700445-100UL 100 µL
$405.00
1/EA
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Western Blotting Western blot analysis of extracts of various cell lines, using Phospho-PPP1R12A-S507 antibody at 1:2,000 dilution or PPP1R12A antibody. HeLa cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes after serum-starvation overnight. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.

 

antibody form affinity isolated antibody
antibody product type primary antibodies
biological source rabbit
clone polyclonal
concentration 1.68 mg/ml
form buffered aqueous solution
mol wt 105
  109
  111
  115
Quality Level 100 
shipped in wet ice
species reactivity human
storage temp. −20°C
technique(s) western blot: 1:500-1:2000
UniProt accession no. O14974 
General description: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC. Rho-associated kinase (Rho-kinase), which is activated by GTP. RhoA, phosphorylated MBS and consequently inactivated myosin phosphatase. Overexpression of RhoA or activated RhoA in NIH 3T3 cells increased phosphorylation of MBS and MLC. Thus, Rho appears to inhibit myosin phosphatase through the action of Rho-kinase. Several transcript variants encoding different isoforms have been found for this gene.
Immunogen: A synthetic phosphorylated peptide around S507 of human PPP1R12A (NP_001137357.1).
Physical form: PBS with 0.02% sodium azide,50% glycerol,pH7.3.
Storage and Stability: Store at -20℃. Avoid freeze / thaw cycles.
WGK Germany WGK 1
Flash Point(F) Not applicable
Flash Point(C) Not applicable
Storage Temp. −20°C
UNSPSC 12352203

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