Anti-Phospho-PLCG2-Y759 antibody produced in rabbit
SIGMA/SAB5700455
Synonym: APLAID; FCAS3; PLC-IV; PLC-gamma-2; PLCG2
Product Type: Chemical
Western Blotting Western blot analysis of extracts of Raji cells, using Phospho-PLCG2-Y759 pAb at 1:2,000 dilution or PLCG2 antibody. Raji cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
Western Blotting Western blot analysis of extracts of Raji cells, using Phospho-PLCG2-Y759 antibody at 1:2,000 dilution or PLCG2 antibody. Raji cells were treated by Pervanadate (1 mM) at 37 °C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 1 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.87 mg/ml |
| form | buffered aqueous solution |
| mol wt | 147 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P16885  |
| General description: | The protein encoded by this gene is a transmembrane signaling enzyme that catalyzes the conversion of 1-phosphatidyl-1D-myo-ino |
| Immunogen: | A synthetic phosphorylated peptide around Y759 of human PLCG2 (NP_002652.2). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |