Anti-Phospho-beta Catenin-S675 antibody produced in rabbit
SIGMA/SAB5700463
Synonym: CTNNB; CTNNB1; MRD19; armadillo; beta Catenin
Product Type: Chemical
Western Blotting Western blot analysis of extracts of C6 cells, using Phospho-β-catenin-S675 antibody at 1:1,000 dilution. C6 cell lysates were treated by CIP at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 180 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.49 mg/ml |
| form | buffered aqueous solution |
| mol wt | 85 |
| 9 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P35222  |
| General description: | The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic phosphorylated peptide around S675 of human beta Catenin (NP_001091679.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |