Anti-Phospho-EIF4G1-S1108 antibody produced in rabbit
SIGMA/SAB5700464
Synonym: EIF-4G1; EIF4F; EIF4G; EIF4G1; EIF4GI; P220; PARK18
Product Type: Chemical
Western Blotting Western blot analysis of extracts of NIH/3T3 cells, using Phospho-EIF4G1-S1108 antibody at 1:1,000 dilution. NIH/3T3 cells were treated by 10% FBS for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 60 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.52mg/ml |
| form | buffered aqueous solution |
| mol wt | 154 |
| 158 | |
| 166 | |
| 171 | |
| 175 | |
| 176 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | Q04637  |
| General description: | The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5′-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. |
| Immunogen: | A synthetic phosphorylated peptide around S1108 of human EIF4G1 (NP_001181875.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |