Anti-Phospho-PRKCQ-T538 antibody produced in rabbit
SIGMA/SAB5700465
Synonym: PRKCQ; PRKCT; nPKC-theta
Product Type: Chemical
Western Blotting Western blot analysis of extracts of Jurkat and HeLa cells, using Phospho-PRKCQ-T538 antibody at 1:2,000 dilution. Jurkat cells were treated by PMA/TPA (200 nM) for 10 minutes. HeLa cells were treated by serum-starvation overnight or treated by PMA/TPA (200 nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 30 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.59 mg/ml |
| form | buffered aqueous solution |
| mol wt | 67 |
| 74 | |
| 81 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | Q04759  |
| General description: | Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. The protein encoded by this gene is one of the PKC family members. It is a calcium-independent and phospholipid-dependent protein kinase. This kinase is important for T-cell activation. It is required for the activation of the transcription factors NF-kappaB and AP-1, and may link the T cell receptor (TCR) signaling complex to the activation of the transcription factors. |
| Immunogen: | A synthetic phosphorylated peptide around T538 of human PRKCQ (NP_006248.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |