Anti-Phospho-PDGFRB-Y1021 antibody produced in rabbit
SIGMA/SAB5700479
Synonym: CD140B; IBGC4; IMF1; JTK12; KOGS; PDGF Receptor beta; PDGFR; PDGFR beta; PDGFR-1; PDGFR1; PDGFRB; PENTT
Product Type: Chemical
Western Blotting Western blot analysis of extracts of NIH/3T3 cells, using Phospho-PDGFRB-Y1021 antibody at 1:1,000 dilution. NIH/3T3 cells were treated by PDGF (100 ng/mL) for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2.9 mg/ml |
| form | buffered aqueous solution |
| mol wt | 123 |
| 37 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, rat, human |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P09619  |
| General description: | This gene encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia. |
| Immunogen: | A synthetic phosphorylated peptide around Y1021 of human PDGFRB (NP_002600.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |