Anti-Phospho-CRKL-Y207 antibody produced in rabbit
SIGMA/SAB5700488
Synonym: CRKL
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using Phospho-CRKL-Y207 antibody at dilution of 1:100 (40x lens).
Western Blotting Western blot analysis of extracts of K-562 cells, using Phospho-CRKL-Y207 antibody at 1:1,000 dilution or CRKL antibody. K-562 cells were treated by CIP (20 μL/400 μL) at 37 °C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 5 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 2 mg/ml |
| form | buffered aqueous solution |
| mol wt | 33 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunohistochemistry: 1:50-1:100 |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P46109  |
| General description: | This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic. |
| Immunogen: | A synthetic phosphorylated peptide around Y207 of human CRKL (NP_005198.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |