Anti-Phospho-EEF2-T56 antibody produced in rabbit
SIGMA/SAB5700495
Synonym: EEF-2; EEF2; EF-2; EF2; SCA26
Product Type: Chemical
Western Blotting Western blot analysis of extracts of various cell lines, using Phospho-EEF2-T56 antibody at 1:1,000 dilution. NIH/3T3 cells were treated by Forskolin (30 μM) for 30 minutes after serum-starvation overnight. C6 cells were treated by Forskolin (10 μM) for 1 hour after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 5 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.44 mg/ml |
| form | buffered aqueous solution |
| mol wt | 95 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | P13639  |
| General description: | This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. |
| Immunogen: | A synthetic phosphorylated peptide around T56 of human EEF2 (NP_001952.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |