Anti-Phospho-PLCB3-S1105 antibody produced in rabbit
SIGMA/SAB5700499
Synonym: PLCB3
Product Type: Chemical
Western Blotting Western blot analysis of extracts of HeLa cells, using Phospho-PLCB3-S1105 antibody at 1:1,000 dilution. HeLa cells were treated by PMA/TPA (200 nM) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 10 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 3.78 mg/ml |
| form | buffered aqueous solution |
| mol wt | 131 |
| 138 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| technique(s) | western blot: 1:500-1:2000 |
| UniProt accession no. | Q01970  |
| General description: | This gene encodes a member of the phosphoinositide phospholipase C beta enzyme family that catalyze the production of the secondary messengers diacylglycerol and inositol 1,4,5-triphosphate from phosphatidylinositol in G-protein-linked receptor-mediated signal transduction. Alternative splicing results in multiple transcript variants. |
| Immunogen: | A synthetic phosphorylated peptide around S1105 of human PLCB3 (NP_000923.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |