Recombinant 2019-nCoV Spike RBD Protein with His tag
SIGMA/SAB5700590
Synonym: 2019-nCoV RBD Protein; NCP-CoV RBD Protein; S glycoprotein Subunit1 RBD Protein; S1-RBD protein; novel coronavirus RBD Protein
Product Type: Chemical
Western Blotting Recombinant 2019-nCoV Spike RBD Protein with His tag was determined by SDS-PAGE with Coomassie Blue, showing a band at 36 kDa.
Recombinant nCoV Spike Immobilized Recombinant 2019-nCoV Spike RBD-His at 2 μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 1.5-9.4 ng/mL.
Purity nCoV Spike The purity of 2019-nCoV Spike RBD Protein with His tag was greater than 95% as determined by SEC-HPLC.
SPR Assay Immobilized human ACE2 on COOH Chip, can bind 2019-nCoV Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay (Nicoya OpenSPR).
| assay | >95% (HPLC) |
| >95% (SDS-PAGE) | |
| form | lyophilized powder |
| impurities | <0.1 EU/μg protein (LAL test) |
| mol wt | 25.9 kDa |
| Quality Level | 100  |
| recombinant | expressed in HEK 293 cells |
| shipped in | wet ice |
| storage temp. | −20°C |
| UniProt accession no. | P0DTC2  |
| Biochem/physiol Actions: | Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Spike RBD-His at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 0.15-4.53 ng/mL. Immobilized human ACE2 on COOH Chip, can bind SARS-COV-2 Spike RBD Protein with an affinity constant of 36.7 nM as determined in a SPR assay. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications. |
| General description: | Recombinant 2019-nCoV Spike RBD-His Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Arg319-Phe541) of 2019-ncov Spike RBD-His (Accession #YP_009724390.1) fused with a 6XHis tag at the C-terminus. |
| Reconstitution: | For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
| Storage and Stability: | Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | >95% (HPLC); >95% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |