Anti-c-Fos antibody produced in rabbit
SIGMA/SAB5700610
Synonym: AP-1; C-FOS; FOS; c-Fos; p55
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded human lung cancer using C-FOS antibody at dilution of 1:150 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse heart using C-FOS antibody at dilution of 1:150 (40x lens).
Western Blotting Western blot analysis of extracts of HepG2 cells, using c-Fos antibody at 1:1,000 dilution.HepG2 cells were treated by serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% BSA. Detection: ECL™ Enhanced Kit (GERPN2235). Exposure time: 5 min.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.94 mg/ml |
| form | buffered aqueous solution |
| mol wt | 28 |
| 36 | |
| 40 | |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | rat, mouse, human |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P01100  |
| General description: | The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 211-380 of human c-Fos (NP_005243.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |