Anti-Lamin A/C antibody produced in rabbit
SIGMA/SAB5700618
Synonym: CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; LDP1; LFP; LGMD1B; LMN1; LMNA; LMNC; LMNL1
Product Type: Chemical
Immunohistochemistry Immunohistochemistry of paraffin-embedded rat brain using Lamin A/C antibody at dilution of 1:200 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded mouse kidney using Lamin A/C antibody at dilution of 1:200 (40x lens).
Immunohistochemistry Immunohistochemistry of paraffin-embedded Human gastric cancer using Lamin A/C antibody at dilution of 1:100 (40x lens).
Immunofluorescence Confocal immunofluorescence analysis of A-431 cells using Lamin A/C Polyclonal antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence Confocal immunofluorescence analysis of HeLa cells using Lamin A/C Polyclonal antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence Confocal immunofluorescence analysis of U-2 OS cells using Lamin A/C Polyclonal antibody at dilution of 1:200. Blue: DAPI for nuclear staining.
Immunofluorescence Immunofluorescence analysis of HeLa cells using Lamin A/C antibody. Blue: DAPI for nuclear staining.
Western Blotting Western blot analysis of extracts of various cell lines, using Lamin A/C antibody at 1:1,000 dilution. HeLa cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours or treated by Paclitaxel (100 nM/mL) at 37 °C for 20 hours. C6 cells were treated by Hydroxyurea (4 mM) at 37 °C for 20 hours or treated by Paclitaxel (100 nM) at 37 °C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL™ Basic Kit (GERPN3004). Exposure time: 5 s.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1.41 mg/ml |
| form | buffered aqueous solution |
| mol wt | 62-74 |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| technique(s) | immunofluorescence: 1:50-1:200 |
| immunohistochemistry: 1:50-1:200 | |
| immunoprecipitation (IP): 1:50-1:200 | |
| western blot: 1:500-1:2000 | |
| UniProt accession no. | P02545  |
| General description: | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. Alternative splicing results in multiple transcript variants. Mutations in this gene lead to several diseases: Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease, and Hutchinson-Gilford progeria syndrome. |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 403-572 of human Lamin C (NP_005563.1). |
| Physical form: | PBS with 0.02% sodium azide,50% glycerol,pH7.3. |
| Storage and Stability: | Store at -20℃. Avoid freeze / thaw cycles. |
| WGK Germany | WGK 1 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |